Nanoparticle description and tracking in virology and drug delivery
Our work aims to understand the structure, assembly and function of biological macromolecule complexes. We embed unstained, unfixed specimens in vitreous ice to preserve their structures. Transmission cryo-electron microscopy is then used to visualize the specimens, but the images recorded are difficult to interpret because they are projections of the specimen degraded by noise. Thus we computationally combine images of the specimen viewed from different angles enabling us to reconstruct a three-dimensional model. Any inherent symmetry present in the structure is also used in this averaging. This method is particularly useful for objects that are too large, unstable or variable to be studied by X-ray crystallography or NMR, such as enveloped viruses.
Research Interests: Assembly and structure determination of viruses, drug delivery, lipids in cells, self-assembly of chlorosomes, cryoelectron microscopy, image processing, crystallography
If you are interested in the Structural biology services offered in Helsinki University, please check out the web pages for cryoEM, for NMR and intein use, for X-ray crystallography, for biocomplex purification
Sarah is one of the “responsible professors” of the Faculty of Biological and Environmental Sciences, she has a regular Monday morning slot for student consultation at 8am. Sign up sheet outside the office (room 2405, Biocenter 1).