Methods: What we do?

Levels of experimentation

-in the field

-in the greenhouse

-in the growth rooms

-in the laboratory

 

Molecular biology

-NGS sequencing

-qPCR

-bioinformatics (mothur)

 

Flux measurements

-CO2, CH4 and N2O

-photosynthesis and soil respiration from microcosms (CO2 exchange)

 

Chemistry

-N chemistry (NO3, NH4, amino acids, total N, TOC-TN, DIN, DON, P-buffer extractable proteins, MSA extractable total proteins)

-with collaborators: amines

 

Enzymology

-MUF- and AMC -based methods (b-glucosidase, cellobiohydrolase, leucine aminopeptidase, xylosidase, chitinase, glucuronidase, phosphatase)

-FITC-casein based: protease

-colorimetric: laccase and manganese peroxidase