What is plant intelligence? and what it is not?

The opinions in this post are those of the author.

The scientific discussion about “plant intelligence” was started by Anthony Trewavas about 10 years ago, culminating with the publication of the book Plant Behaviour and Intelligence. The use of the word “intelligence” for plant was then and still remains controversial. On the other hand phenomena described and the experiments used as support are not specially controversial.

In recent times the use of the term “plant intelligence” has reached the daily press and science popularization books in addition to videos in the internet. Much of this material is not only controversial in the meaning given to the word “intelligence” but also in the quality or strength of the evidence used. This has led, in my view, to confusion and misinterpretations of what has been actually demonstrated with experiments compared to what are tentative hypotheses. Continue reading “What is plant intelligence? and what it is not?”

How did we work out that UVR8 is a solar-UV-B plus UV-A photoreceptor?

What were our findings?

We reported in two recent research papers and an update review, that functional UVR8 is required for the perception by plants of solar UV-radiation with wavelengths shorter than approximately 340 nm, which includes the whole UV-B band plus the shorter wavelengths in the UV-A band. In sunlight, cryptochromes are required for the perception by plants of blue light and the longer wavelengths within the UV-A band leading to changes in gene expression. In sunlight cryptochrome-mediated signalling is driven mostly by violet and blue light with wavelength longer than 400 nm. In comparison wavelengths between 350 nm and 400 nm of solar radiation seem to play only a minor role in the regulation of gene expression.

This is an important step forward in our understanding of the perception of different wavelengths of sunlight by plants as the former accepted view was that UVR8 is a UV-B photoreceptor that participated only in the perception of UV-B radiation while all wavelengths of UV-A radiation were perceived by cryptochromes and the other UV-A/Blue photoreceptors, phototropins and ZTL.

Continue reading “How did we work out that UVR8 is a solar-UV-B plus UV-A photoreceptor?”

Brighter times for our growth chambers

The latest LEDs from Nichia and other suppliers are game changers. I designed a replacement light source for our Aralab Fitoclima 1200 growth chambers and Nikolai Belevich (Biotechnology Institute) assembled a prototype before the start of the pandemic. This post was written time ago, but I am publishing it now that the final design of the light sources is ready for use after extensive testing. Nikolai Belevich also assembled the six light boxes for three growth chambers based on the final design, to which he also contributed.

In this post I briefly describe the prototype and some of the steps that led to the final design. I also discuss how the latest LED components, icnluding some specifically designed for horticulture, have qualitatively changed lighting possibilities in growth chambers and rooms.

Continue reading “Brighter times for our growth chambers”

Perception of solar UV radiation by plants: photoreceptors and mechanisms

Our Update article has been published in July’s issue of Plant Physiology. It summarizes the current state of knowledge including the new understanding and insights brought to light by the research by our group in Helsinki, done with the help of several collaborators.

Title: Perception of solar UV radiation by plants: photoreceptors and mechanisms

Authors: Neha Rai, Luis O. Morales, Pedro J. Aphalo

Neha Rai is now at the University of Geneva in Roman Ulm’s lab.

Luis O. Morales is at Örebro University leading his own research group.

DOI (open access): https://doi.org/10.1093/plphys/kiab162

Abstract:

About 95% of the ultraviolet (UV) photons reaching the Earth’s surface are UV-A (315–400 nm) photons. Plant responses to UV-A radiation have been less frequently studied than those to UV-B (280–315 nm) radiation. Most previous studies on UV-A radiation have used an unrealistic balance between UV-A, UV-B, and photosynthetically active radiation (PAR). Consequently, results from these studies are difficult to interpret from an ecological perspective, leaving an important gap in our understanding of the perception of solar UV radiation by plants. Previously, it was assumed UV-A/blue photoreceptors, cryptochromes and phototropins mediated photomorphogenic responses to UV-A radiation and “UV-B photoreceptor” UV RESISTANCE LOCUS 8 (UVR8) to UV-B radiation. However, our understanding of how UV-A radiation is perceived by plants has recently improved. Experiments using a realistic balance between UV-B, UV-A, and PAR have demonstrated that UVR8 can play a major role in the perception of both UV-B and short-wavelength UV-A (UV-Asw, 315 to ∼350 nm) radiation. These experiments also showed that UVR8 and cryptochromes jointly regulate gene expression through interactions that alter the relative sensitivity to UV-B, UV-A, and blue wavelengths. Negative feedback loops on the action of these photoreceptors can arise from gene expression, signaling crosstalk, and absorption of UV photons by phenolic metabolites. These interactions explain why exposure to blue light modulates photomorphogenic responses to UV-B and UV-Asw radiation. Future studies will need to distinguish between short and long wavelengths of UV-A radiation and to consider UVR8’s role as a UV-B/UV-Asw photoreceptor in sunlight.

Figure 5 from the paper at Plant Physiology web site

FBES/OEB/SenPEP weather station

We have a weather station at our experimental field that I look after. It started collecting data in 2015 and in 2020 it had a large upgrade. There is a full description of the equipment and measurements at https://viikki-stn.r4photobiology.info/.

Photograph of the station
The weather station in November 2020

Differently to standard meteorological stations data are logged for most variables at 1 min intervals, as means of 12 measurements taken at 5 s intervals. Hourly and daily summaries are also stored.

Currently the station logs more than 40 variables, a few of them from multiple sensors. Special emphasis is on solar radiation, with measurements of photon irradiance for UV-B, UV-A, blue, red and far-red, and global energy irradiance. Differently to measurements of  erythemal, i.e., biologically effective UV radiation more commonly available, UV-A and UV-B irradiances are measured separately and without applying a spectral weighting function.

With the most recent upgrade we started measurements of the profile of soil temperature, water content and electrical conductivity. Surface temperature is measured on vegetation and/or bare soil with non-contact (infrared) sensors.

The usual air temperature, water vapour pressure, atmospheric pressure, wind speed, wind direction and rainfall are also recorded. The data are available on request. The radiation data for the winter period are less reliable than for the growth season, and there are also some gaps in past winters for all variables.

 

Cryptochromes and stomatal opening

Our recent paper, included in Fang Wang’s thesis:

Fang Wang, T Matthew Robson, Jorge J Casal, Alexey Shapiguzov, Pedro J Aphalo (2020) Contributions of cryptochromes and phototropins to stomatal opening through the day. Functional Plant Biology, 47, 226-238. DOI: https://doi.org/10.1071/FP19053.

The role of phototropins in stomatal opening in response to blue light in well documented in the literature. Reports of a role for cryptochromes in this response have been few, and to some extent contradictory. Most studies on the daily patterns of stomatal opening date from the time when well described photoreceptor mutants were not yet available, so using these mutants was expected to reveal new features of stomatal responses.

Continue reading “Cryptochromes and stomatal opening”

NEW BOOK: Learn R: As a Language

My book Learn R: As a Language was published some weeks ago by Chapman & Hall / CRC in the “The R Series”.

ISBN 9780367182533

As I a side note, I have typeset the whole book using R and LaTeX using the same approach as for reproducible data analyses. All code examples are run and their textual and graphical outputs generated each time the camera ready PDF is built. This ensures that all code is functional and that all output is up-to-date at the time the PDF is generated. In the spirit of openness and reproducibility, the source files used for generating the book PDF are available through the public Git repository at https://bitbucket.org/aphalo/learnr-book-crc/.

R for photobiology packages

I have published through CRAN a suite of R packages for calculation and plotting tasks commonly needed in photobiology. Additional information and on-line documentation are available at The R for Photobiology Website.

This is open source, free to use and modify software that aims to make it easier for all photobiologists to do calculations correctly and play with example data. In addition, the example data facilitates the production of original illustrations for use in teaching and text books.

Continue reading “R for photobiology packages”

UVR8 is an UV-B and UV-A photoreceptor

Our two recent papers:

Neha Rai, Susanne Neugart, Yan Yan, Fang Wang, Sari M Siipola, Anders V Lindfors, Jana Barbro Winkler, Andreas Albert, Mikael Brosché, Tarja Lehto, Luis O Morales, Pedro J Aphalo (2019) How do cryptochromes and UVR8 interact in natural and simulated sunlight? Journal of Experimental Botany, 70, 4975–4990. https://doi.org/10.1093/jxb/erz236

Neha Rai, Andrew O’Hara, Daniel Farkas, Omid Safronov, Khuanpiroon Ratanasopa, Fang Wang, Anders V. Lindfors, Gareth I. Jenkins, Tarja Lehto, Jarkko Salojärvi, Mikael Brosché, Åke Strid, Pedro J. Aphalo, Luis O. Morales (2020) The photoreceptor UVR8 mediates the perception of both UV‐B and UV‐A wavelengths up to 350 nm of sunlight with responsivity moderated by cryptochromes. Plant, Cell & Environment, https://doi.org/10.1111/pce.13752

In these two recent publications we have shown that UVR8, previously described as an ultravioltet-B (UV-B, 280-315 nm) photoreceptor, in sunlight functions both as an ultraviolet-A (UV-A, 315-400 nm) and UV-B photoreceptor. Although UVR8 presents maximal absorption at the boundary between ultraviolet-C (UV-C, <280 nm) and UV-B, the shape of the solar spectrum in the ultraviolet region, characterized by a very steep slope, allows the UVR8 protein to absorb nearly as many UV-A photons as UV-B photons, and obviously no photons in the UV-C as they are not present in sunlight at ground level.

Normalized spectral absoorbance of UVR8 protein in vitro (From Rai et al.. 2020).

Continue reading “UVR8 is an UV-B and UV-A photoreceptor”

Measuring campaign in the Alps

I joined a field measuring campaign organized by my collaborator T. Matthew Robson (see Matt’s CanSEE website for information on the research project) with the participation of José Ignacio García Plazaola and Beatriz Fernandez-Marin from the University of the Basque-Country.

Matthew described the aim of our work as:

By characterising the patterns of response to UV radiation in terms of the photoprotection and UV-screening of plants across a diversity of species, we hope to better understand how and why these response evolved and what environmental cues underpin their induction.

We spent the last weeks of May the at 2100 m a.s.l. in the Alps at the Jardin Botanique du Lautaret measuring solar radiation and the responses of plants to it. I did some measurements of solar radiation but spent most of the time photographing plants and lichens to record their optical properties in the ultraviolet-A, visible and near-infrared regions of the spectrum.

Villar-d’Arêne, French Alps, 2100 m a.s.l.

Several of the photographs I took of site, crew, plants and lichens available at my photography website in a post published earlier today (as I have the server set up for easy creation of galleries). These photographs are stored at Flickr.

Matthew has also written a post about the trip and project in his blog.