Publications

2019

KOVALCHUK A, ZENG Z, GHIMIRE RP, KIVIMÄENPÄÄ M, RAFFAELLO T, LIU M, MUKRIMIN M, KASANEN R, SUN H, JULKUNEN-TIITTO R, HOLOPAINEN JK and  ASIEGBU FO (2019). Dual RNA-seq analysis provides new insights into interactions between Norway spruce and necrotrophic pathogen Heterobasidion annosum s.l. BMC Plant Biology 19:2.  doi:10.1186/s12870-018-1602-0

TERHONEN E, BLUMENSTEIN K, KOVALCHUK A and ASIEGBU FO (2019). Forest tree microbiomes and associated fungal endophytes: Roles and impact on forest health.   Forests 10(1), 42; doi:10.3390/f10010042

ZENG Z, WU J, KOVALCHUK A, RAFFAELLO T, WEN Z, LIU M  and  ASIEGBU FO (2019). Genome-wide DNA methylation and transcriptomic profiles in the lifestyle strategies and asexual development of the forest fungal pathogen Heterobasidion parviporum. Epigenetics   doi:10.1080/15592294.2018.1564426

 

2018

KOVALCHUK A, MUKRIMIN M, ZENG Z, RAFFAELLO T, LIU M, KASANEN R, SUN H and  ASIEGBU FO (2018). Mycobiome and metatranscriptomic analysis of asymptomatic and symptomatic Norway spruce trees naturally infected by the conifer pathogen Heterobasidion spp. Environmental Microbiology Reports 10(5):532-541.  doi:10.1111/1758-2229.12654

LINNAKOSKI R, KASANEN R, LASAROV I, MARTTINEN T, OGHENEKARO AO, SUN H, ASIEGBU FO, WINGFIELD MJ, HANTULA J, HELIÖVAARA K (2018). Cadophora margaritata sp. nov. and other fungi associated with the longhorn beetles Anoplophora glabripennis and Saperda carcharias in Finland. Antonie van Leeuwenhoek 111(11):2195-2211. doi:10.1007/s10482-018-1112-y

MUKRIMIN M, KOVALCHUK A, NEVES LG, JABER E, HAAPANEN M, KIRST M, and ASIEGBU FO (2018). Genome-wide exon-capture approach identifies genetic variants of Norway spruce genes associated with susceptibility to Heterobasidion parviporum infection. Frontiers in Plant Science 9:793. doi: 10.3389/fpls.2018.00793

REN F, KOVALCHUK A, MUKRIMIN M, LIU M, ZENG Z, GHIMIRE RP,  KIVIMÄENPÄÄ M, HOLOPAINEN JK, SUN H, ASIEGBU FO (2018). Tissue microbiome of Norway spruce affected by Heterobasidion-induced wood decay. Microbial Ecology doi:10.1007/s00248-018-1240-y

ZENG Z, SUN H, VAINIO EJ, RAFFAELLO T, KOVALCHUK A, MORIN E, DUPLESSIS S AND ASIEGBU FO (2018). Intraspecific comparative genomics of isolates of the Norway spruce pathogen (Heterobasidion parviporum) and identification of its potential virulence factors. BMC Genomics 19: 220 doi:10.1186/s12864-018-4610-4

ZENG Z, RAFFAELLO T, LIU M & ASIEGBU FO (2018). Co-extraction of genomic DNA and total RNA from recalcitrant woody tissues for next-generation sequencing (NGS) studies. Future Scienc OA.    doi:10.4155/fsoa-2018-0026

 

2017

JABER E, KOVALCHUK A, Raffaello T, Keriö S,  TEERI T AND ASIEGBU FO (2017). A gene encoding Scots Pine antimicrobial protein Sp-AMP2 (PR-19) confers increased tolerance against Botrytis cinerea in Transgenic Tobacco.   Forests 9(1), 10; doi:10.3390/f9010010

JAEYOUNG CHOI, GIR-WON LEE, KI-TAE KIM, JONGBUM JEON, NICOLAS DÉTRY, HSIAO-CHE KUO, HUI SUN, FRED O. ASIEGBU AND YONG-HWAN LEE (2017). Comparative analysis of genome sequences of the conifer tree pathogen, Heterobasidion annosum s.s. Genomics Data (Data in brief) 14: 106–113

LINNAKOSKI R, SUGANO J, JUNTTILA S, PULKKINEN P, ASIEGBU FO, FORBES KM (2017). Effects of water availability on a forestry-fungi pathosystem: strain-specific variation in disease severity. Scientific Reports,  7: 13501,  DOI:10.1038/s41598-017-13512-y

KOVALCHUK A, ZHU L, KERIÖ S AND ASIEGBU FO (2017). Differential responses of Scots pine stilbene synthase and chalcone synthase genes to Heterobasidion annosum infection. Forest Pathology, 47 (4),  DOI: 10.1111/efp.12348

JARKKO SALOJÄRVI, OLLI-PEKKA SMOLANDER, KAISA NIEMINEN, SITARAM RAJARAMAN, OMID MOHAMMADI, PEZHMAN SAFDARI, AIRI LAMMINMÄKI, JUHA IMMANEN, TIANYING LAN, JAAKKO TANSKANEN, PASI RASTAS, ALI AMIRYOUSEFI, BALAMURALIKRISHNA JAYAPRAKASH, JUHANA KAMMONEN, RISTO HAGQVIST, GUGAN ESWARAN, MARKKU AALTO, VIIVI HELENA AHONEN, FRED O. ASIEGBU, JD BARAJAS-LOPEZ, DANIEL BLANDE, OLGA BLOKHINA, TIINA BLOMSTER, SUVI BROHOLM, MIKAEL BROSCHÉ, FUQIANG CUI, CHRIS DARDICK, PAULA ELOMAA, SACHA ESCAMEZ, KURT V. FAGERSTEDT, HIROAKI FUJII, ADRIEN GAUTHIER, PETER J. GOLLAN, PAULIINA HALIMAA, KRISTIINA HIMANEN, COURTNEY HOLLENDER, , SAIJALIISA KANGASJÄRVI, LEILA KAUPPINEN, COLIN T. KELLEHER, SARI KONTUNEN-SOPPELA, J. PATRIK KOSKINEN, ANDRIY KOVALCHUK, SIRPA KÄRENLAMPI, ANNA KÄRKÖNEN, KEAN-JIN LIM, JOHANNA LEPPÄLÄ, LEE MACPHERSON, JUHA MIKOLA, KATRIINA MOUHU, ELINA OKSANEN, KIRK OVERMYER, E. TAPIO PALVA, LEILA PAZOUKI, VILLE PENNANEN, TUULA PUHAKAINEN, PÉTER POCZAI, BOY J.H.M. POSSEN, MATLEENA PUNKKINEN, MOONA RAHIKAINEN, MATTI ROUSI, RAILI RUONALA, CHRISTIAAN VAN DER SCHOOT, JUAN ALONSO SERRA, ALEXEY SHAPIGUZOV, MAIJA SIERLA, TIMO P. SIPILÄ, SUVI SUTELA, TEEMU H. TEERI, ARJA IRMELI TERVAHAUTA, ALEKSIA VAATTOVAARA, JORMA VAHALA, LIDIA VETCHINNIKOVA, ANNIKKI WELLING ,MICHAEL WRZACZEK, ENJUN XU, LARS PAULIN, ALAN SCHULMAN, MARTIN LASCOUX, VICTOR A. ALBERT, PETRI AUVINEN, YKÄ HELARIUTTA, JAAKKO KANGASJÄRVI (2017) Adaptive Landscape and Population Genomics of Silver Birch.    Nature Genetics, 49: 904–912, doi:10.1038/ng.3862

LINNAKOSKI R, FORBES KM, WINGFIELD MJ, PULKKINEN P, ASIEGBU FO (2017). Testing projected climate change conditions on the Endoconidiophora polonica / Norway spruce pathosystem shows fungal strain specific effects. Frontiers Plant Science 8: 883,  DOI: 10.3389/fpls.2017.00883

RAFFAELLO T AND  ASIEGBU FO (2017). Small secreted proteins from the necrotrophic conifer pathogen  Heterobasidion annosum s.l. (HaSSP) induces strong cell death in Nicotiana benthamiana. Scientific Reports 7: 8000, DOI:10.1038/s41598-017-08010-0

2016

SUN H, SANTALAHTI M, PUMPANEN J, KÖSTER K, BERNINGER F, RAFFAELLO T, ASIEGBU FO,  HEINONSALO J (2016) Bacterial community structure and function shift across a boreal forest fire chronosequence. Scientific Report (Nature) 6: 32411  (DOI: 10.1038/srep32411)

BARAL B, KOVALCHUK A AND ASIEGBU FO (2016). Genome organisation and expression profiling of ABC protein-encoding genes in Heterobasidion annosum s.l. complex. Fungal Biology, 120 (3), 376–384 (doi:10.1016/j.funbio.2015.11.005)

OGHENEKARO AO, RAFFAELLO T, KOVALCHUK A AND ASIEGBU FO (2016). De novo assembly and transcriptomic profiling of Rigidoporus microporus genes expressed during saprotrophic growth on rubber wood. BMC Genomics 17:234 DOI: 10.1186/s12864-016-2574-9

SUN H, TERHONEN E, KOVALCHUK A, TUOVILA H, CHEN H, OGHENEKARO A, HEINONSALO J, KOHLER A, KASANEN R, VASANDER H, ASIEGBU FO (2016). Dominant tree species and soil type affect fungal community structure in a boreal peatland forest. Applied and Environmental Microbiology 82(9): 2632-43 (doi: 10.1128/AEM.03858-15)

OGHENEKARO AO, OMORUSI VI AND ASIEGBU FO (2016). Defence-related gene expression of Hevea brasiliensis clones in response to the white rot pathogen, Rigidoporus microporus. Forest pathology, DOI: 10.1111/efp.12260

TERHONEN E, SIPARI N AND ASIEGBU FO (2016). Inhibition of phytopathogens by fungal root endophytes of Norway spruce. Biological Control 99:53–63.

 

2015

SUN H, SANTALAHTI M, PUMPANEN J,KOSTER K, BERNINGER F, RAFFAELLO T, JUMPPONEN J, ASIEGBU FO, HEINONSALO J (2015). Fungal community shifts in structure and function across a boreal forest fire chronosequence. Applied and Environmental Microbiology 81 (22): 7869-7880 (September 2015, doi: 10.1128/AEM.02063-15 )

CHEN H, QUINTANA GONZÁLEZ J, KOVALCHUK A, UBHAYASEKERA W, ASIEGBU FO (2015). Cerato-platanin-like protein HaCPL2 from Heterobasidion annosum sensu stricto induces cell death in Nicotiana tabacum and Pinus sylvestris. Fungal Genetics & Biology 84: 41–51

KOVALCHUK A, KOHLER A, LEE YH, MARTIN F AND ASIEGBU FO (2015). Diversity and evolution of ABC proteins in mycorrhiza-forming fungi. BMC Evolutionary Biology 12/2015; 15(1). DOI:10.1186/s12862-015-0526-7

CHOI J, KIM KT, HUH A, KWON S, HONG C, ASIEGBU FO, JEON J AND LEE YH (2015). dbHiMo: a web-based epigenomics platform forhistone-modifying enzymes. Databases (The Journal of Biological Databases and Curation) Database, 2015, 1–10 doi: 10.1093/database/bav052

LUNDÉN K, DANIELSSON M, DURLING MB, IHRMARK K, NEMESIO GM STENLID J, ASIEGBU FO AND ELFSTRAND M (2015). Transcriptional responses associated with virulence and defence in the interaction between Heterobasidion annosum s.s. and 30-year old Norway spruce trees. PLoS ONE 10(7): e0131182. doi:10.1371/journal.pone.0131182

OGHENEKARO AO, DANIEL G AND ASIEGBU FO (2015). The saprotrophic wood-degrading abilities of Rigidoporus microporus. Silva Fennica vol. 49 no. 4 article id 1320. http://dx.doi.org/10.14214/sf.1320

KERIÖ S, NIEMI S, HAAPANEN M, DANIEL G AND ASIEGBU FO (2015). Infection of Picea abies clones with a homokaryotic isolate of Heterobasidion parviporum under field conditions. Canadian Journal of Forest Research 45: 226–234, dx.doi.org/10.1139/cjfr-2014-0247

KOVALCHUK A, KERIÖ S, JABER E, RAFFAELLO T, LORENZ W, RAJENDRA GHIMIRE, DEAN R, HOLOPAINEN JK AND ASIEGBU FO (2015). Activation of defence pathways in Scots pine bark after feeding by Pine Weevil (Hylobius abietis). BMC Genomics 16:352 DOI: 10.1186/s12864-015-1546-9

SILVA X, TERHONEN E, SUN H, KASANEN R, HELIÖVAARA K, JALKANEN R AND ASIEGBU FO (2015). Comparative analyses of fungal biota carried by the pine shoot beetle (Tomicus piniperda L.) in Northern and Southern Finland. Scandinavian Journal of Forest Research, DOI: 10.1080/02827581.2015.1031824

2014

DÉTRY N, CHOI J, KUO HS, ASIEGBU FO, VALKONEN JPT AND LEE YH (2014). In silico sequence analysis reveals new characteristics of fungal NADPH oxidase genes. Mycobiology 42(3) : 241-248

CHOI J, KIM KT, JEON J, WU J, SONG H, ASIEGBU FO AND LEE YH (2014). funRNA: a fungi-centered genomics platform for genes encoding key components of RNAI. BMC Genomics 15 (Suppl 9):S14 doi:10.1186/1471-2164-15-S9-S14

CHIAKI HORI; TAKUYA ISHIDA; KIYOHIKO IGARASHI; MASAHIRO SAMEJIMA; HITOSHI SUZUKI; EMMA MASTER; PATRICIA FERREIRA; FRANCISCO J RUIZ-DUENAS; BENJAMIN HELD; PAULO CANESSA; LUIS F LARRONDO; MONIKA SCHMOLL; IRINA S DRUZHININA; CHRISTIAN P KUBICEK; JILL GASKELL; PHIL KERSTEN; FRANZ ST. JOHN; JEREMY GLASNER; GRZEGORZ SABAT; SANDRA SPLINTER BONDURANT; KHAJAMOHIDDIN SYED; JAGJIT YADAV; ANTHONY C MGBEAHURUIKE; ANDRIY KOVALCHUK; FRED O ASIEGBU; GERALD LACKNER; DIRK HOFFMEISTER; HUI SUN; ERIKA LINDQUIST; KERRIE BARRY; ROBERT RILEY; IGOR V GRIGORIEV; BERNARD HENRISSAT; URSULA KÜES; RANDY M BERKA; ANGEL T MARTINEZ; SARAH F COVERT; ROBERT A BLANCHETTE; DANIEL CULLEN (2014). Analysis of the Phlebiopsis gigantea genome, transcriptome and secretome gives insight into its pioneer colonization strategies of wood. PLOS Genetics 10(12): e1004759. doi:10.1371/journal.pgen.1004759

JABER  E, XIAO C  AND ASIEGBU FO  (2014). Comparative pathobiology of Heterobasidion annosum during challenge on Pinus sylvestris and Arabidopsis roots: analysis of defensin gene expression in the pathosystems. Planta 239: 717 – 733 (http://dx.doi.org/10.1007/s00425-013-2012-z) .

SUN H, TERHONEN  E, KOSKINEN K, PAULIN  L, KASANEN  R, ASIEGBU FO (2014). Bacterial diversity and community structure along different peat soils in boreal forest. Applied Soil Ecology 74: 37 – 45.

SUN H, TERHONEN  E,  KASANEN  R, ASIEGBU FO (2014). Diversity and community structure of bacteria associated with decaying wood in boreal forest. Geomicrobiology 31(4): 315 –  324

TERHONEN E, KERIÖ S, SUN H,  AND ASIEGBU FO (2014). Endophytic fungi of Norway spruce roots in boreal pristine mire, drained peatland and mineral soil and inhibitory effect on Heterobasidion parviporum in vitro. Fungal Ecology 9: 17 – 26.

OGHENEKARO AO, MIETTINEN O,  OMORUSI VI, EVUEH G,  FARID  MA,  GAZIS  R, AND ASIEGBU FO (2014). Characterization of Rigidoporus species associated with white rot of Para rubber (Hevea brasiliensis) plantations.  Fungal Biology 118(5-6): 495-506, http://dx.doi.org/10.1016/j.funbio.2014.04.001

JAEYOUNG CHOI, NICOLAS DÉTRY, KI-TAE KIM, FRED O ASIEGBU, JARI P T VALKONEN AND YONG-HWAN LEE (2014). Fungal peroxidase database for comparative genomics. BMC Microbiology  14:117  doi:10.1186/1471-2180-14-117

HSIAO-CHE KUO, JAEYOUNG CHOI, HUI SUN, FRED O. ASIEGBU, JARI PT VALKONEN & YONG-HWAN LEE (2014). A secret life of Neurospora crassa. Scientific Reports 4 (5135): 1 – 6, doi:10.1038/srep05135

2013

SUN H, TERHONEN E, KOSKINEN K, PAULIN L, KASANEN R AND ASIEGBU FO (2013) The impacts of treatment with biocontrol fungus (Phlebiopsis gigantea) on  bacteria biota of Norway spruce stumps. Biological Control, 64: 238–246 http://dx.doi.org/10.1016/j.biocontrol.2012.11.015,

ARNERUP J, NEMESIO-GORRIZ M, LUNDÉN  K, ASIEGBU FO, STENLID J AND ELFSTRAND M (2013) The primary module in Norway spruce defence signalling against H. annosum s.l. seems to be jasmonate-mediated signalling without antagonism of salicylate-mediated signalling. Planta  237: 1037–1045 (DOI: 10.1007/s00425-012-1822-8)

KOVALCHUK A, KERIÖ S, JABER E, OGHENEKARO A, RAFFAELLO T AND ASIEGBU FO (2013) Antimicrobial defences and resistance of forest trees: challenges and perspectives in a genomic era. Annual Review of Phytopathology vol 51: 221 – 244.

RAFFAELLO T  AND ASIEGBU FO (2013)  Evaluation of potential reference genes for use in gene expression studies in the conifer pathogen Heterobasidion annosum.  Molecular Biology Reports  40: 4605–4611 (DOI 10.1007/s11033-013-2553-z).

MGBEAHURUIKE AC,   KOHLER A AND ASIEGBU FO (2013) Expression analysis of the impact of culture filtrate from the biocontrol agent  (Phlebiopsis gigantea) on the conifer pathogen (Heterobasidion annosum s.s.) transcriptome. Microbial Ecology 66: 669 – 681 (DOI 10.1007/s00248-013-0255-7).

KOVALCHUK A, LEE YH AND ASIEGBU FO (2013)  Diversity and evolution of ABC proteins in basidiomycetes. Mycologia 105:1456-1470  (doi: 10.3852/13-001).

CHEN H,  KOVALCHUK A AND ASIEGBU FO (2013) Distribution and bioinformatic analysis of cerato-platanin protein family in Dikarya. Mycologia 105:1479-1488 (doi: 10.3852/13-115).

MGBEAHURUIKE AC,  KOVALCHUK A AND ASIEGBU FO (2013) Comparative genomics and evolutionary analysis of three species of wood degrading fungi. Mycologia 105:1471-1478 (doi: 10.3852/13-077)

JAMES TY, SUN S, LI W, HEITMAN J, KUO HC, ASIEGBU FO OLSON Å (2013) Polyporales genomes reveal the genetic architecture underlying tetrapolar and bipolar mating systems Mycologia 105:1374-1390  (doi: 10.3852/13-162 )

TERHONEN E, SUN H, BUÉE M, KASANEN R, PAULIN L AND ASIEGBU F.O (2013) Effects of the use of biocontrol agent (Phlebiopsis gigantea) on fungal communities of Picea abies stumps. Forest Ecology & Management 310: 428 – 433.

SUN H, TERHONEN  E, KOSKINEN K, PAULIN  L, KASANEN  R, ASIEGBU FO (2014) Bacterial diversity and community structure along different peat soils in boreal forest. Applied Soil Ecology 74: 37 – 45.

MGBEAHURUIKE AC, KOVALCHUK A, CHEN H, UBHAYASEKERA W, ASIEGBU FO (2013) Evolutionary analysis of hydrophobin gene family in two wood-degrading basidiomycetes, Phlebia brevispora and Heterobasidion annosum s.l. BMC Evolutionary Biology (1):240. DOI: 10.1186/1471-2148-13-240

RAFFAELLO T, CHEN H, KOHLER A AND ASIEGBU FO (2013) Transcriptomic profiles of Heterobasidion annosum under abiotic stresses and during saprotrophic growth in bark, sapwood and heartwood.  Environmental Microbiology 13:240. DOI: 10.1186/1471-2148-13-240

SUN H, TERHONEN  E,  KASANEN  R, ASIEGBU FO (2013) Diversity and community structure of bacteria associated with decaying wood in boreal forest. Geomicrobiology (in press)

JABER  E AND ASIEGBU FO  (2013) Comparative pathobiology of Heterobasidion annosum during challenge on Pinus sylvestris and Arabidopsis roots: analysis of defensin gene expression in the pathosystems. Planta (In press).

2012

Heller G, Lunen K, Finlay R, Asiegbu FO  & Elfstrand M (2012)  Expression analysis of Clavata1-like and Nodulin21-like genes from Pinus sylvestris during ectomycorrhiza formation.  Mycorrhiza 22:271?277:DOI: 10.1007/s00572-011-0402-2

Åke Olson, Andrea Aerts, Fred Asiegbu, Lassaad Belbahri, Ourdia Bouzid, Anders Broberg, Björn Canbäck, Pedro M. Coutinho, Dan Cullen, Kerstin Dalman, Giuliana Deflorio, Linda T.A. van Diepen, Christophe Dunand, Sébastien Duplessis, Mikael Durling, Paolo Gonthier, Jane Grimwood, Carl Gunnar Fossdal, David Hansson, Bernard Henrissat, Ari Hietala, Kajsa Himmelstrand, Dirk Hoffmeister, Nils Högberg, Timothy Y. James, Magnus Karlsson, Mårten Lind, Annegret Kohler, Ursula Kües, Yong-Hwan Lee, Yao-Cheng Lin, Erika Lindquist, Vincent Lombard, Susan Lucas, Karl Lundén, Emmanuelle Morin, Claude Murat, Jongsun Park, Tommaso Raffaello, Pierre Rouzé, Asaf Salamov, Jeremy Schmutz, Halvor Solheim, Jerry Ståhlberg, Heriberto Vélëz, Ronald P. de Vries, Ad Wiebenga, Steve Woodward, Igor Yakovlev, Matteo Garbelotto, Francis Martin, Igor V. Grigoriev, Jan Stenlid (2012) Trade-off between wood decay and parasitism: Insights from the genome of a fungal forest pathogen. New Phytologist. http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2012.04128.x/full

Mgbeahuruike, A.C., Karlsson, M., Asiegbu, F.O. (2012) Differential expression of two hydrophobin genes (Pgh1 and Pgh2) from the biological control agent Phlebiopsis gigantea. Fungal Biology DOI: 10.1016/j.funbio.2012.03.001

Raffaello T, Keriö S, Asiegbu FO (2012) Role of the HaHOG1 MAP Kinase in Response of the Conifer Root and But Rot Pathogen (Heterobasidion annosum) to Osmotic and Oxidative Stress. PLoS ONE 7(2): e31186. doi:10.1371/journal.pone.0031186

2011

Terhonen E, Marco T, Sun H, Jalkanen R, Kasanen R, Vuorinen M, Asiegbu FO (2011) The effect of latitude, season and needle-age on the mycota of Scots pine (Pinus sylvestris) in Finland. Silva Fennica 45(3): 301 – 317.

Kasanen R, Terhonen E, Huuskonen S, Sun H, Uotila A. (2011) High infection rate of residual conifer stumps by Heterobasidion species in an area with assumed low infection pressure. Scandinavian Journal of Forest Research DOI: 10.1080/02827581.2011.586366.

Eastwood DC, Floudas D, Binder M, Majcherczyk A, Schneider P, Aerts A, Asiegbu FO, Baker SE, Barry K, Bendiksby M, Blumentritt M, Coutinho PM, Cullen D, de Vries RP, Gathman A, Goodell B, Henrissat B, Ihrmark K, Kauserud H, Kohler A, LaButti K, Lapidus A, Lavin JL, Lee YH, Lindquist E, Lilly W, Lucas S, Morin E,  Murat C, Oguiza JA, Park J, Pisabarro AG, Riley R, Rosling A, Salamov A, Schmidt O, Schmutz J, Skrede I, Stenlid J, Wiebenga A, Xie X, Kües U, Hibbett DS, Hoffmeister D, Högberg N, Martin F,  Grigoriev IV and Watkinson SC (2011) The Plant Cell Wall-Decomposing Machinery Underlies the Functional Diversity of Forest Fungi. Science 333: 762 – 765, DOI: 10.1126/science.1205411.

Heller G, Lundén K, Finlay RD, Asiegbu FO and Elfstrand M (2011) Expression analysis of Clavata1-like and Nodulin21-like genes from Pinus sylvestris during ectomycorrhiza formation. Mycorrhiza, DOI: 10.1007/s00572-011-0402-2.

Hui Sun, Lars Paulin, Edward Alatalo and Fred O. Asiegbu (2011) Response of living  tissues of Pinus sylvestris to the saprotrophic biocontrol fungus Phlebiopsis gigantea. Tree Physiology 31: 438-451.

Sanjeewani Sooriyaarachchi, Emad Jaber, Adrian Suárez Covarrubias, Wimal Ubhayasekera, Frederick O. Asiegbu and Sherry L. Mowbray (2011) Expression and beta-glucan binding properties of Scots pine (Pinus sylvestris L.) antimicrobial protein (Sp-AMP). Plant Molecular Biology 77 (1-2):  33 –  45.

Vainio, E., Keriö, S. & Hantula, J. (2011) Description of a new putative partitivirus infecting the conifer pathogenic fungus Heterobasidion parviporum with resemblance to Heterobasidion annosum P-type partitivirus. Archives of Virology 156(1): 79-86.

Mgbeahuruike AC, Sun H, Fransson P, Kasanen R, Daniel G, Karlsson M  and Asiegbu FO (2011) Differential screening of Phlebiopsis gigantea isolates for traits associated with biocontrol of the conifer pathogen Heterobasidion annosum. Biological Control 57: 118-129.

Niskanen A-M, Kärkkäinen K and Pasonen H (2011) Comparison of variation in adaptive traits between wild-type and transgenic silver birch (Betula pendula) in a field trial. Tree Genetics & Genomes 7(5): 955-967.

Jinrong Lu (2011) The effects of abiotic and biotic factors on somatic embryogenesis and seedlings of Pinus sylvestris (L.). Ph.D thesis, University of Helsinki, Helsinki,Finland. URN:ISBN:978-952-10-4292-8

Lu J, Vahala J, Pappinen A (2011) Involvement of ethylene in somatic embryogenesis in Scots pine (Pinus sylvestris L.). Plant Cell, Tissue and Organ Culture (In press).

2010

Lilja A, Poteri M, Petäistö R-L, Rikala R, Timo Kurkela, Kasanen R (2010) Fungal Diseases in Forest Nurseries in Finland. Silva Fennica 44: 525−545.

Chen J, Tang B, Chen H, Yao Q, Huang X, et al. (2010) Different Functions of the Insect Soluble and Membrane-Bound Trehalase Genes in Chitin Biosynthesis Revealed by RNA interference. PLoS ONE 5(4): e10133. doi:10.1371/journal.pone.0010133

Jaber E, Thiele K, Kindzierski V, Loderer C, Rybak K, Jürgens G, Mayer U, Söllner R, Wanner G, Assaad FF (2010) A putative TRAPPII tethering factor is required for cell plate assembly during cytokinesis in Arabidopsis. New Phytol 187: 751–763.

Lu J, Aronen T, Pappinen A, Asiegbu FO (2010) Response of somatic embryos of Scots pine to fungal cell wall elicitors. Forest Pathology. doi: 10.1111/j.1439-0329.2010.00641.x

2009

Lehr N., Adomas A.,Asiegbu F.O., Hampp R. & Tarkka M.T. (2009) WS-5995 B, an antifungal agent  inducing differential gene expression in the conifer pathogen Heterobasidion annosum. Applied Microbiology & Biotechnology 85 (2): 347 – 358.

Pasonen, H.-L., Lu, J., Niskanen, A.-M., Seppänen, S.-K.,  Rytkönen, A., Raunio, J., Pappinen, A., Kasanen, R. and Timonen, S. (2009) Effects of sugar beet chitinase IV on root-associated root community of silver birch in a field trial. Planta 230: 973 – 983.

Sun, H., Korhonen, K, Hantula, J., Asiegbu, F.O. & Kasanen, R.  (2009) Use of a breeding approach for improving biocontrol efficacy of Phlebiopsis gigantea strains against Heterobasidion infection of Norway spruce stumps. FEMS Microbiology Ecology 69:266-273.

Sun, H., Korhonen, K., Hantula, J.& Kasanen, R. (2009) Variation in properties of Phlebiopsis gigantea related to biocontrol against infection by Heterobasidion spp. in Norway spruce stumps. Forest Pathology 39:133–144.

2008

La Porta, N. Capretti, P. Thomsen, I.M., Kasanen, R., Hietala, A.M. & Von Weissenberg, K. (2008) Forest pathogens with higher damage potential due to climate change in Europe. Canadian Journal of Plant Pathology 30:177-195.

Adomas A.,  Heller, G., Li G., CraigD., ChuT.,  Olson A., ZylL.V.,  WolfingerR., SederoffR.,  Finlay R., StenlidJ.,  &  Asiegbu F.O. (2008) Comparative analysis of transcript abundance in Pinus sylvestris roots after challenge with a saprotrophic, pathogenic or mutualistic fungus. Tree Physiology 28: 885-897.

Heller, G, Adomas A.,  Li G., Adomas A.,  CraigD., Chu T.,   Zyl L.V.,  Wolfinger R., Sederoff R.,  Finlay R., StenlidJ.,  &  Asiegbu F.O. (2008) Transcriptional analysis of Pinus sylvestris roots challenged with the ectomycorrhizal fungus Laccaria bicolor. BMC Plant Biology 8: 19 –

Lohtander K, Pasonen H-L, Palva T, Pappinen A, Rikkinen J. (2008) Phylogeny of chitinases and implications for estimating horizontal gene transfer from chitinase transgenic silver birch (Betula pendula). Environmental Biosafety Research 7(4): 227-239.

Lunden K., Eklund M., Finlay R., Stenlid J., Asiegbu F.O. (2008) Heterologous array analysis in Heterobasidion spp. of cDNA arrays with probe from mycelium of S, P or F types. Journal of Microbiology Methods 75: 219 – 224.

Niskanen, A.-M., Stenvall, N., Pakkanen, A. and Pulkkinen, P.  (2008) Comparison of growth and stem form characteristics of Pinus sylvestris clones and seedlings of the same origin in a 10-year field trial. Scandinavian Journal of Forest Research 23: 424 – 290.

Pasonen H-L, Vihervuori L, Seppänen S-K, Lyytikäinen-Saarenmaa P, Ylioja T, von Weissenberg K, Pappinen A (2008) Field performance of chitinase transgenic silver birch (Betula pendula): Growth and adaptive traits. Trees – Structure and Function 22: 413-421.

Vihervuori L, Pasonen H-L, Lyytikäinen-Saarenmaa P. (2008) Density and composition of insect population in a field trial of chitinase transgenic and wild-type silver birch (Betula pendula) clones. Environmental Entomology 37 (6): 1582-1591.

2007

Adomas A. & Asiegbu F.O. 2007.  Analysis of organ specific responses of Pinus sylvestris to shoot (Gremmeniella abietina) and root (Heterobasidion annosum) pathogens. Physiological and Molecular plant Pathology 69: 140 – 152.

AdomasA.,  Li G., CraigD., Chu T., Heller G., OlsonA., ZylL.V.,  WolfingerR., SederoffR.,  Dean R.A., FinlayR., StenlidJ. &  Asiegbu F.O. 2007. Transcriptome analyses of early events during interactions of  Scots pine root tissues with pathogenic fungi.  Tree Physiology 27: 1441–1458.

Seppänen S-K, Pasonen H-L, Vauramo S, Vahala J, Toikka M, Kilpeläinen I, Setälä H, Teeri T, Timonen S, Pappinen A 2007. Decomposition of the leaf litter and mycorrhiza forming ability of silver birch with a genetically modified lignin biosynthesis pathway. Applied Soil Ecology 36: 100-106.

2006

Adomas A., Eklund M., Johansson M., Asiegbu F.O. 2006. Identification and analysis of differentially expressed cDNA’s during non-self competitive interaction between Phlebiopsis gigantea and Heterobasidion parviporum. FEMS Microbiology Ecology 57: 26 – 39.

Li G., OsbornE. J. & Asiegbu F.O. 2006. A macro-array expression analysis of novel cDNA’s vital for growth initiation and primary metabolism during development of Heterobasidion parviporum conidiospores. Environmental Microbiology 8: 1340 – 1360.

StepniewskaS., MankaM., AsiegbuF.O. 2006. Anastomosis grouping, pathogenicity and population structure of Rhizoctonia spp. isolates from forest nurseries in Poland. Forest Pathology 36: 97 – 109.

Vauramo S, Pasonen H-L, Pappinen A, Setälä H. 2006. Decomposition of leaf litter from chitinase transgenic silver birch (Betula pendula) and effects on decomposer populations in a field trial. Applied Soil Ecology 32: 338-349.

2005

AsiegbuF.O., Nahalkova J. & Li G. 2005. Pathogen inducible cDNAs from the     interaction of the root rot fungus Heterobasidion annosum (P-type) with its conifer host (Pinus sylvestris). Plant Science  168: 365 – 372.

Asiegbu F.O., Adomas A. & Stenlid J. 2005. Pathogen Profile: The root and butt rot fungus-Heterobasidion annosum Molecular Plant Pathology 6(4): 395 – 409.

Pasonen H-L, Degefu Y, Brumós J, Lohtander K, Pappinen A, Timonen S, Seppänen S-K. 2005. Transgenic Betula pendula expressing sugar beet chitinase IV forms normal ectomycorrhizae with Paxillus involutus in vitro. Scandinavian Journal of Forest Research 20: 385-392.

2004

Abu SM, Li G & Asiegbu FO 2004. Identification of Heterobasidion annosum genes  involve in conidiospore germination  under varying culture conditions. FEMS Microbiology Letters 233: 205 – 213.

Asiegbu F.O., Abu S., Stenlid J. & Johansson M. 2004. Molecular characterization of laccase genes of the conifer pathogen. Mycological Research 108: 136 – 148.

Asiegbu F.O., Choi W., Jeong Y.S. & Dean R.A. 2004. Cloning, sequencing and functional analyses of Magnaporthe grisea MVP1 gene, a hex-1 homolog encoding putative woronin body protein. FEMS Microbiology Letters  230: 85 – 90.

Johansson S.M., Lundgren L.N. & Asiegbu F.O. 2004. Initial reactions in sapwood of Norway spruce and Scots pine after wounding and infection by Heterobasidion parviporum and H. annosum. Forest Pathology 34 (3): 197 – 210.

Kasanen, R., Hantula, J., Ostry, M. E., Pinon, J. & Kurkela, T. 2004. North American populations of Entoleuca mammata are genetically more variable than populations in Europe. Mycological Research 108:766-774.

Kasanen, R., Hantula, J., Vuorinen, M., Stenlid, J., Solheim, H. & Kurkela, T. 2004. Migrational capacity of Fennoscandian populations of Venturia tremulae. Mycological Research 108:64-70.

Li G  & Asiegbu FO 2004 Use of Scots pine seedling roots as an experimental model to investigate gene expression during interaction with the conifer pathogen Heterobasidion annosum (P-type). Journal of Plant Research 117 (2): 155 – 162.

Li G & Asiegbu FO 2004. Induction of Pinus sylvestris PsACRE,, a homolog of Avr9/Cf-9 rapidly elicited defence related gene following infection with the root rot fungus Heterobasidion annosum. Plant Science 167: 535 – 540.

Niskanen, A.-M., Lu, J., Seitz, S., Keinonen, K., von Weissenberg, K. and Pappinen, A. 2004. Effect of parent genotype on somatic embryogenesis in Scots pine (Pinus sylvestris). Tree Physiology 24: 1259 – 1265.

Parantainen A, Pasonen H-L. 2004. Pollen-pollen interactions in vitro in Scots pine (Pinus sylvestris). Scandinavian Journal of Forest Research 19: 1-7.

Pasonen H-L, Seppänen SK, Degefu Y, Rytkönen A, von Weissenberg K, Pappinen A. 2004. Field performance of chitinase transgenic silver birches (Betula pendula): Resistance to fungal diseases. Theoretical and Applied Genetics 109: 562-570.

Pietarinen P, Pasonen H-L. 2004. Pollen performance and male fitness in an anemophilous, monoecious tree, Betula pendula. Canadian Journal of Botany 82: 1284-1291.

2003

ASIEGBU FO, CHOI W, LI G, NAHALKOVA J & DEAN RA. 2003. Isolation of a novel antimicrobial peptide gene from Pinus sylvestris. FEMS Microbiology Letters 228: 27 – 31.

2002

JOHANSSON M,  ASIEGBU FO & PRATT J. 2002. Stump treatment against Heterobasidion annosum with urea: possible modes of action. Forest Ecology and Management- 157 (1-3): 87 – 100.

KACPRZAK M, ASIEGBU FO, DANIEL G, MANKA M & STENLID J. 2002. In vitro Scots pine seedling root colonization by damping-off fungi in the presence of a Trichoderma harzianum isolate. Phytopathologia. polonica. 23: 5 – 15.

HANTULA J,  KASANEN R, KAITERA J &  MORICCA S. 2002. Analyses of genetic variation suggest that pine rusts Cronartium flaccidum and Peridermium pini belong to the same species. Mycological Research 106: 203 – 209

Kasanen R, Hantula J & Kurkela T. 2002. Neofabraea populi in Hybrid Aspen Stands in Southern Finland 17: 391-397

2001

KACPRZAK M, ASIEGBU FO, DANIEL, G. JOHANSSON M , STENLID J. & MANKA, M. 2001. Differential resistance of conifer species (European Larch, Norway spruce, Scots pine) to infection by necrotrophic parasites. European Journal of Plant Pathology 107 (2): 191 – 207.

NAHALKOVA J, ASIEGBU FO, DANIEL G, HRIB J, VOOKOVA B, PRIBULOVA, B & GEMEINER P. 2001. Isolation and characterization of a Pinus nigra lectin (PNL) during interaction with the necrotrophs-Heterobasidion annosum and Fusarium avanaceum. Physiological and Molecular Plant Pathology  59: 153 – 163

KASANEN R, KAITERA J & HANTULA J. 2001. The genetic composition of Peridermium pini and Cronartium flaccidum cankers on Scots pine as revealed by two multi-allelic loci. Forest Pathology 30: 221 – 231.

KASANEN R, HANTULA J & KURKELA T. 2001. The occurrence of an undescribed species of Venturia in blighted shoots of Populus tremula Mycological Research 105: 338 – 343.

2000

ASIEGBU FO  2000. Effects of ethanol, cell wall phenolics and carbohydrates on in vitro growth of necrotrophic fungi- Heterobasidion annosum and Fusarium avenaceum. Journal of Basic Microbiology 3: 139 – 148.

ASIEGBU FO  2000. Adhesion  and development of the root rot fungus (Heterobasidion annosum) on conifer tissues: effects of spore and host surface constituents. FEMS Microbiology Ecology  33(2): 101 – 110.

KACPRZAK, M. ASIEGBU FO, DANIEL G & MANKA, M. 2000. Changes in root structure of Scots pine seedlings infected with Rhizoctonia solani observed under fluorescence and electron microscopes.  Phytopathologia polonica 19: 127 – 140.

ASIEGBU FO 2000.  Genetic transformation of the root rot fungus Heterobasidion annosum by particle bombardment. Forestry Studies 34; 9 – 16.

 KACPRZAK, M. ASIEGBU FO, & MANKA, M. 2000. Acidity and peroxidase activity change in Scots pine and Norway spruce seedling roots after infection with Rhizoctonia solani and Fusarium spp. SYLWAN 4: 99 –103.

KACPRZAK, M. ASIEGBU FO, & MANKA, M. 2000. Cell wall lignification in roots of Scots pine, Norway spruce and European larch seedlings after infection with Rhizoctonia solani and Fusarium spp. SYLWAN 4: 105 – 112.

1999

JOHANSSON M, DENEKAMP M & ASIEGBU FO 1999.  Production and isozyme pattern of extracellular laccase in the S and P intersterility groups of the root pathogen Heterobasidion annosum. Mycological Research  103 (3): 365 – 371.

ASIEGBU FO, JOHANSSON M & STENLID J. 1999. Reactions of Pinus sylvestris (Scots pine) root tissues to the presence of mutualistic, saprotrophic and necrotrophic micro-organisms. Journal of Phytopathology 147: 257 – 264.

ASIEGBU FO, KACPRZAK, M. DANIEL, G. JOHANSSON M, STENLID J. & MANKA, M. 1999. Biochemical interactions of conifer seedling roots with Fusarium  spp Canadian Journal of Microbiology 45: 923 – 935.

1998

DANIEL G, ASIEGBU FO & JOHANSSON M. 1998. Saprophytic wood decaying capabilities of the S and P intersterility groups of Heterobasidion annosum. Mycological Research 102: 991 – 998.

JOHANSSON M, LUNDGREN  L & ASIEGBU FO 1998. Differential phenol-induced laccase activity of the S and P intersterility groups of the conifer root pathogen Heterobasidion annosum. Microbiological Research 153: 71 – 80.

ASIEGBU FO, JOHANSSON M,   HUTTERMANN A & WOODWARD S 1998. Biochemistry of the host-parasite interaction. In Heterobasidion annosum in European Forests: Prospects for Control. Edited by S. Woodward, J. Stenlid, R. Karjalainen & A. Huttermann. CAB International Publishers, London pp167 – 193.

1997

ASIEGBU FO, JOHANSSON M & DANIEL G. 1997. Cytopathological response reactions of Picea abies roots to infection by the necrotroph- Fusarium avenaceum (Fr.) Sacc.)  Journal of Phytopathology  145: 239 – 244.

KASANEN R. 1997. Aeciospores of Cronartium flaccidum, C. ribicola and Endocronartium pini show no differences in morphology. European Journal of Forest Pathology 27: 251-260

1996

ASIEGBU FO, PATERSON A & SMITH JE 1996.  Inhibition of cellulose saccharification and  glyco-lignin attacking enzymes of five lignocellulose degrading fungi by ferulic acid. World Journal of Microbiology and Biotechnology  12 (1): 16 – 21.

ASIEGBU FO,  DANIEL G & JOHANSSON M. 1996. Cellular Interaction between the saprotroph Phlebiopsis gigantea and non-suberized roots of Picea abies. Mycological Research 100(4): 409 – 417.

ASIEGBU FO, PATERSON A & SMITH JE 1996. The effects  of co-fungal cultures and supplementation with carbohydrate adjuncts on lignin biodegradation and substrate digestibility.  World Journal of Microbiology and Biotechnology 12: 273 – 279.

ASIEGBU FO, LÖNNEBORG A,  & JOHANSSON M. 1996. Chitins and glucans detected in cell walls of the oomycetous fungus-Pythium dimorphum European Journal of Forest Pathology  6: 315 – 321.

PAPPINEN A, KASANEN R, MÖYKKYNEN T & VON WEISSENBERG K 1996. Fast methods for DNA extraction for PCR analyses from Heterobasidion annosum mycelium and Endocronartium pini aeciospores. European Journal of Forest Pathology 26: 81-88

PAPPINEN A, KASANEN R & WEISSENBERG K  1996. Identification of multiple Endocronartium pini infections in Scots pine with PCR. European Journal of Forest Pathology 26: 183-191

PAPPINEN A, KASANEN R & WEISSENBERG K  1996. Identification of multiple Endocronartium pini infections in Scots pine with PCR. European Journal of Forest Pathology 26: 183-191

1995

ASIEGBU FO,  DENEKAMP M, DANIEL G & JOHANSSON M. 1995. Immunocytochemical localisation of pathogenesis related proteins in roots of Norway spruce infected with Heterobasidion annosum. European Journal of Forest Pathology 25 (3): 161 – 170.

ASIEGBU FO,  DANIEL G & JOHANSSON M. 1995. Infection and disintegration of vascular tissues of non-suberized roots of spruce by Heterobasidion annosum and use of antibodies for characterising infection. Mycopathologia 129: 91 – 101.

ASIEGBU FO, MORISSON I, PATERSON A & SMITH JE 1995. Effect of plant cell wall phenolics and fungal metabolites on acetate and methane production under in vitro rumen conditions.  Journal of General and Applied Microbiology 41: 475 – 485.

1994

ASIEGBU FO, MORISSON I, PATERSON A & SMITH JE 1994. Analyses of fungal fermentation of lignocellulosic substrates using continuos culture rumen simulation. Journal of General and Applied  Microbiology 40: 305 – 318.

ASIEGBU FO,  DANIEL G & JOHANSSON M. 1994. Defence related reactions of seedling roots of Norway spruce to infection by Heterobasidion annosum (Fr.) Bref. Physiological and Molecular Plant Pathology 45: 1 – 19.

1993

ASIEGBU FO,  DANIEL G & JOHANSSON M. 1993. Studies on infection of Norway spruce roots by Heterobasidion annosum. Canadian Journal of   Botany 71: 1552 – 1561.

 

 

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